PreOmics

“Using the APP96 is enhancing our productivity and throughput by saving us lots of time in preparing high quality samples for total proteome quantifications, interaction proteomics and protein quality checkups.”

“[…] ENRICHplus is changing the way we do plasma proteomics. On Bruker’s timsTOF Pro we could achieve on average 3,800 protein identifications per sample with a throughput of 60 samples per day while only using 20ul of plasma. We never before achieved such depth with a single run of plasma, now enabling us to detect tissue leakage proteins and other low abundant potential biomarkers for cancer and neurodegenerative diseases. […] ENRICHplus is a complete, efficient and game changing platform for deep plasma proteomics.”

“In our Core, we always aim to stay ahead of advancements, which is why gaining access to ENRICH™ technology has been a game-changer. With ENRICH-iST, we doubled our plasma protein identification capabilities. Then came ENRICHplus, which took things even further. On average, we now identify around 3,600 plasma protein groups in single shot experiments, a 5.3-fold increase compared to our standard workflow using iST-BCT for neat plasma.”

“[…] In the context of clinical cohort digitalization, our protocols need to be robust, highly reproducible and automatable. When processing plasma samples, we regularly used ENRICH-iST since it fulfilled all our requirements. We recently evaluated the newly released ENRICHplus kit from PreOmics. In our hands, the identification of proteins with the ENRICHplus kit clearly outperformed the standard ENRICH-iST. We also transferred both protocols to the Opentrons FLEX and found that automation was easily feasible with both kits.”

“The ENRICHplus kit has significantly advanced our research on human plasma by allowing us to identify an impressive number of close to 5,000 cumulative protein groups, a level of detection that we had not previously achieved. We hereby especially value the reliability, reproducibility, affordability and consistency of the kit, with the majority of proteins detected in 50% of the 126 samples tested. This makes ENRICHplus ideal for large cohort studies, especially when combined with the sensitivity and speed of Bruker’s nanoELUTE2 and timsTOF HT.”

“The BeatBox platform has significantly streamlined our proteomics workflows, particularly for challenging FFPE and tissue samples. Its robustness, ease of automation, and compatibility with Evosep One coupled to either Bruker's timsTOF or Thermo's Astral systems have made BeatBox essential for high-throughput and reproducible clinical proteomics.”

“ENRICHplus has substantially boosted our plasma proteomics capability, efficiently depleting high-abundance proteins and enabling deep proteome profiling. Its seamless integration with Evosep One chromatography provides consistent, high-quality results, making ENRICHplus an excellent choice for sensitive biomarker discovery in large-scale clinical studies.”

“The new PepSep® column has proven to be robust and consistently delivers reproducible chromatography. It produces sharper, more symmetrical peaks with no observable tailing, resulting in a 7–8% increase in identifications. Combined with the convenience of interchangeable emitters and competitive pricing, it has become our go-to column. It’s essentially plug-and-play.”

“We are truly amazed by the performance of PepSep® columns, which have become indispensable for us in achieving remarkable high-throughput proteomics. Working seamlessly alongside our Bruker timsTOF Pro mass spectrometer, PepSep columns enable us to utilize higher nanoflow rates with minimal back pressure elevation. This translates to quicker analysis times and a substantial boost in sample throughput.””

“iST technology improves proteomics throughput and data depth. Our focus is on plasma metabolomics and proteomics in large-scale cohorts in Japan using the MxP® Quant 500 and ENRICH kit family. Together, they enable more sensitive and robust analyses, supporting large-scale multi-omics studies.””

“Using PepSep Advanced columns on our timsTOF Pro coupled to a nanoElute2, we routinely identify over 5,600 protein groups and 57,000+ unique peptides per run from just 10 ng of HeLa digest — with inter-run CVs for retention time consistently below 1.3% across replicates. The combination delivers a robust, reproducible workflow that we now rely on as the backbone of our clinical proteomics service.””

“At the Helsinki Proteomics Center, we have benchmarked essentially all major plasma depletion/enrichment strategies on the market. When depth matters, P2-iST consistently comes out on top. For us it has been a game changer – it puts robust, deep plasma proteomics into the hands of any lab without expensive proprietary hardware. The reproducibility and depth we see with P2-iST on our Orbitrap Astral platform makes it ideal for medium scale clinical and translational plasma studies.”

“Recently, we have evaluated the performance of the new PreOmics® kit, the ENRICH-iST, for biofluids analysis. It improved the proteome depth by 4-fold for plasma and 1.6-fold for CSF compared to nondepleted samples. It is surprisingly easy to use, allowing us to prepare 96 samples simultaneously.”

“BeatBox® has become an indispensable tool in our laboratory at the Max Planck Institute of Biochemistry, Munich. It is our go-to instrument for tissue homogenization, whether dealing with needle biopsy samples from clinics or bulk tissues.”

“We tested several options for biomarker discovery on plasma samples, but many were very long and tedious. With the novel ENRICH technology, we have a fast, efficient, and reliable enrichment workflow to get deeper into the proteome from human plasma and serum. We would strongly recommend the kit to other human plasma and serum users.”